The role of effector Ssc1 in Sclerotinia Sclerotiorum and pathogenicity of Botrytis cinerea in the early infection stages

In this experiment, the action mechanism of gene Ssc1 was studied in process interaction with plants through heterologous expression, subcellular localization and fluorescent PCR technology. It found that could enhance pathogenicity Botrytis cinerea expression. Fusing promoter, SP CTP GFP expressing tobacco, it fusion protein be secreted into plant cells located chloroplasts. Trypan blue staining fluorescence detection early stage after inoculation areas outside scab, detected tobacco leaves despite trypan being negative. Additionally, proved by quantitative highly expressed infection stages. Taken together, these results indicated effector an important pathogenic factor, which locate chloroplasts mainly play role during between S. sclerotiorum plants.   Key words: Sclerotinia sclerotiorum; effector; chorismate mutase; expression; localization.